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TIG - Tolerance Laboratory
Professor Steve Cobbold (Senior Scientist)Steve Cobbold's Home Page
Steve is interested in the mechanisms of peripheral tolerance, and ways of generating tolerance as a therapeutic procedure. Most recently he has been working on identifying the molecular mechanisms of linked suppression and infectious tolerance. He has recently identified an important role for nutrient sensing, particularly of essential amino acids, as a mechanism of immune regulation and stability of regulatory T cells.
He does quite a lot of analysis of T-cell subsets by up to 9 colour FACS, sorting and cloning, with Liz Adams (also you should see Steve if you wish to use the TIG FACSCalibur with 96 well plate autosampler facilities.)
Steve also recently corordinated a successful Departmental application for an ImageStreamX machine, which is essentially a high resolution fluorescent microscope combined with a flow cytometer, which can provide quantitative analysis of fluorescent images in up to 10 simultaneous colours and collect up to 2000 images per second with a resolution down to 0.3micron/pixel.
He has also written various software for the lab, including a complete suite of sofware for SAGE (and microarray) analysis, some of which can be downloaded.
Sue is also looking after the TaqMan machines for quantitative real-time RT-PCR. Th1, Th2, Tr1, Th9 and foxP3+ Treg subsets ex vivo, using ELISAs, bioassays and cytoplasmic staining with 4 colour FACS analysis for cytokines including IFN-gamma, IL-4, IL-10, IL-9 and TNF-alpha. She is also looking at the role of mast cells in tolerance and their relationship to Tr1 and Th9 cells. She continues to determine, together with Steve, the requirements for inducing foxP3 and regulatory activity by anti-CD4 blockade in vitro and in vivo.
Room 214.20.32 (Note change)
Last Updated 16th January 2013 by Steve Cobbold