Percentage of total events being sorted | 3+ | 8+ | 15+ | 24+ | 40+ | 60+ |

Actual maximum sort rate (cells/sec)* | 20 | 50 | 100 | 200 | 300 | (400) |

Number of sorted cells required ** | Time | to | Sort | in | minutes. | |

20,000 | 20 | 8 | 4 | 2 | 1 | 1 |

50,000 | 50 | 18 | 9 | 5 | 3 | 2 |

100,000 | 100 | 34 | 17 | 9 | 6 | 4 |

200,000 | 200 | 68 | 34 | 17 | 11 | 9 |

500,000 | -- | 170 | 85 | 42 | 28 | 21 |

1,000,000 | -- | -- | -- | 85 | 56 | 42 |

5,000,000 | -- | -- | -- | -- | 280 (4h:40m) | 210 (3h:30m) |

10,000,000 | Forget | it | use | a | FACSVantage | or MoFlow! |

* the maximum sort rate is limited by the abort rate increasing as a smaller proportion of events is being sorted. This means that a total event rate of 2000/second usually generates the fastest sort rate regardless of the proportion being sorted. All figures are base on the EXCLUSION MODE.

** these cell numbers assume 100% recovery - in practice expect 60-90% yields after spinning down.

Remember that you will need 1 x 50ml tube for every 9 minutes of sort time regardless of the sort rate. So if you really want to try and sort 5,000,000 cells over 4 hours 40 mins then have 32 coated tubes ready!