Antibodies produced in Bioreactors

Amounts of monoclonal antibodies made in the Dunn School "Home Made" Cartridges

(Period of 34 Months to Sept 1997)

Antibody	Specificity	Parent		Isotype		Amount (g)


YTS 105.18   	Mouse CD8a    	Y3/Ag1.2.3     	Rat IgG2a      	14.7
YTC 182.2    	Human CD8a    	Y3/Ag1.2.3     	Rat IgG2b      	8.4
YTS 177.9.6.1	Mouse CD4     	Y3/Ag1.2.3     	Rat IgG2a      	7.2
YTA 3.1.2    	Mouse CD4     	Y3/Ag1.2.3     	Rat IgG2b      	6.8
11B11        	Mouse IL-4    	Mouse?Sp2/0?   	Rat IgG1       	6.3
YTS 191.1.2  	Mouse CD4     	Y3/Ag1.2.3     	Rat IgG2b      	5.8
YTC 141.1    	Human CD8a    	Y3/Ag1.2.3     	Rat IgG2b      	5.6
YTS 156.7.7  	Mouse CD8b    	Y3/Ag1.2.3     	Rat IgG2b      	10.2
YTS 169HL    	Mouse CD8a    	Y3/Ag1.2.3     	Rat IgG2b      	5.3
MTF 171      	Mouse CD8a    	Y3 transfected 	Human IgG1(ag) 	4.3
C178-20      	Mouse IL-12   	Mouse?         	Rat IgG1       	3.2
YCATE 55.9   	Canine CD8    	Y3/Ag1.2.3     	Rat IgG1       	3.2
KT3.1.1      	Mouse CD3     	Y3?            	Rat IgG2a      	3.0
MEL 14       	Mouse Selectin	P3X63Ag8       	Rat IgG2a      	2.7
TFCD18Ag     	Human CD18    	NS0 transfected	Human IgG1(ag) 	2.4
JES5-2A5     	Mouse IL-10   	Mouse?         	Rat IgG1       	2.8
PK 136       	Mouse NK1.1   	Mouse Sp2/0    	Mouse IgG2a    	0.7
30H12        	Mouse Thy-1.2 	Mouse?X63?     	Rat IgG2b      	0.5
MR1          	Mouse CD40L   	Mouse?         	Hamster IgG    	2.0
NORIG 7.16   	Rat IgG2b     	NS0            	Mouse IgG      	4.4
XMG1.2       	Mouse IFN-g   	NS0            	Mouse IgG      	3.4
YID 13.9     	CAMPATH-Id    	Y3/Ag1.2.3     	Rat IgG1       	Approx 5g
YKIX 302.9   	Canine CD4    	Y3/Ag1.2.3     	Rat IgG2a      	4.5
YTH 655.5    	Human CD2     	Y3/Ag1.2.3     	Rat IgG2b      	1.2
S4B6         	Mouse IL-2    	Mouse?X63?     	Rat IgG1       	2.4
HB32         	Mouse H-2Ek   	Mouse?X63?     	Mouse IgG      	4.1
YMSM 59      	Mouse CD30    	Y3/Ag1.2.3     	Rat IgM        	2.5
YMSM 636     	Mouse CD30    	Y3/Ag1.2.3     	Rat IgG2b      	1.2
YLAG 77      	Mouse LAG-3   	Y3/Ag1.2.3     	Rat IgG2b      	0.8
C15.6.7      	Mouse IL-12   	Mouse?         	Rat IgG1       	1.6


	Total (g)	Approx. 146.4g and 30 different antibodies
So far we are making an average of roughly 4gm of a new antibody every month - well done Sue!
It should be noted that we have not so far had much success with the following cell lines, mostly due to their poor levels of secretion or instability in large scale culture (even after re-cloning etc):

Desire: poor secretion (only produces well if mycoplasma positive!)
37.51 (hamster anti-mouse CD28): unstable - still trying to reclone
Aglycosyl hIgG1 CD3 transfectant: poor secretion
mCTLA4-IG transfectants: poor secretion
HB32 (mouse anti-mouse Ek): originally unstable but solved by recloning

NB: Overall final yield of purified antibody is roughly 18mg per litre of medium. In terms of medium costs, this equates to a current average of less than 120 pounds per gram.

Last updated 22.10.96