Raff Lab Selected Publications
2012
Roque, H., Wainman, A., Richens, J., Kozyrska, K., Franz, A., Raff, JW. Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. J. Cell Sci. 2012 125 5881-5886|PDF|
Baumbach, J., Levesque, MP., Raff, JW. Centrosome loss or amplification does not dramatically perturb global gene expression in Drosophila. Bio. Open 2012 doi: 10.1242/bio.20122238 |PDF|
Reschen, RF., Colombie, N., Wheatley, L., Dobbelaere, J., St Johnston, D., Ohkura, H., Raff, JW. Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRC. Bio. Open 2012 1(5) 422-429 |PDF|
2011
Cottee, MA., Raff, JW., Lea, SM., and Roque, H. 2011. SAS-6 oligomerization: the key to the centriole? Nat. Chem. Biol. 7(10) 650-653 |PDF|
2010
Stevens, N., Dobbelaere, J., Brunk, K., Franz, A., and Raff, JW. 2010. Drosophila Ana2 is a conserved centriole duplication factor JCB. 188(3) 313-323 |PDF|
Stevens, N., Roque, H., and Raff, JW. 2010. DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement Dev. Cell 19(6) 913 - 919 |PDF|
Conduit, PT., Brunk, K., Dobbelaere, J., Dix, CI., Lucas, EP., Raff, JW. 2010. Centrioles regulate centrosome size by controlling the rate of Cnn incorporation into the PCM Curr. Biol. 20(24) 2178 - 2186 |PDF|
Conduit, PT., and Raff, JW. 2010. Cnn dynamics drive centrosome size asymmetry to ensure daughter centriole retention in drosophila neuroblasts Curr. Biol. 20(24) 2187 - 2192 |PDF|
2009
Stevens, N., Dobbelaere, J., Wainman, A., Gergely, F., Raff, JW. 2009. Ana3 is a conserved protein required for the structural integrity of centrioles and basal bodies. JCB. 187(3) 355-363|PDF|
Nigg, E and Raff, JW 2009. Centrioles, Centrosomes, and Cilia in Health and Disease. Cell. 139(4) pp. 663 - 678. |PDF|.
2008
Dobbelaere, J., Josue, F., Suijkerbuijk, S., Baum, B., Tapon, N., and Raff, J.W. 2008. A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila. PloS Biology. 6, e224. |PDF|
Basto, R., Brunk, K., Vinadogrova, T., Peel, N., Franz, A., Khodjakov, A., and Raff, J.W. 2008. Centrosome amplification can initiate tumourigenesis in flies. Cell. 133, 1032-1042. |PDF|.
2007
Dix, C., and Raff, J.W. 2007. Drosophila Spd-2 is essential for PCM recruitment to the sperm centriole, but not for centriole duplication. Curr. Biol. 17, 1759-64.|PDF|
Stevens, N.R., Raposo, A.S.F., Basto, R., St Johnston, D., and Raff, J.W. 2007. From stem cell to embryo without centrioles. Curr. Biol. 21, 1498-1503. |PDF|
Lucas, E., and Raff, J.W. 2007. Maintaining the proper connection between the centrioles and the PCM requires Drosophila Centrosomin. J. Cell Biol. 178, 725-732.|PDF|
Peel, N., Stevens, N.R., Basto, R., and Raff, J.W. 2007. Overexpressing centriole replication proteins in vivo induces centriole overduplication and de novo formation. Curr. Biol. 17, 834-843.|PDF|
Basto, R., Gergely, F., Draviam, V.M., Ohkura, H., Liley, K., Raff, J.W. 2007. Hsp90 is required to localize Cyclin B and Msps/Ch-Tog to the mitotic spindle in Drosophila and humans. J. Cell Sci. 120, 1278-1287.|PDF|
2006
Basto, R., Lau, J., Vinogradova, T., Gardiol, A., Wood, G., Khodhjakov, A., and Raff, J.W. 2006. Flies without centrioles. Cell 125, 1375-1386.|PDF|
2005
Barros, T., Kinoshita K., Hyman, A.A., and Raff, J.W. 2005. Aurora-A activates D-TACC/Msps complexes exclusively at centrosomes to stabilise centrosomal MTs. J. Cell. Biol. 170, 1039-1046.|PDF|
Kinoshita, K., Noetzel, T.L., Pelletier, L., Mechtler, K., Drechsel, D.N., Schwater, A., Lee, M., Raff, J.W., and Hyman, A.A. 2005. Aurora A phosphorylation of TACC3/Maskin is required for centrosome dependent microtubule assembly in mitosis. J. Cell Biol. 170, 1047-1055.|PDF|
Chodagam, S., Royou, A., Whitfield, W., Karess, R., and Raff J.W. 2005. The centrosomal protein CP190 regulates myosin function during early Drosophila development. Curr. Biol. 15, 1308-1313.|PDF|
2004
Martinez-Campos, M., Basto, R., Baker, J., Kernan, M., and Raff., J.W. 2004. The Drosophila pericentrin-like protein is essential for cilia/flagella function but appears to be dispensable for mitosis. J. Cell Biol. 165, 673-683.|PDF|
Raff, J. W. 2004. Centrosomes in a developing organism: lessons from Drosophila. In: “Centrosomes in Development and Disease”. Ed. Nigg, E.A. Wiley-VCH.
Butcher, R.D.J., Chodagam, S., Basto, R., Wakefield, J., Henderson, D.S., Raff, J.W., and Whitfield, W.G.F. 2004. The Drosophila centrosome-associated protein CP190 is essential for viability, but not for cell division J. Cell Sci. 117, 1191-1199.
2003
Raff, J. W. 2003. Genome maintenance. Nature. 423, 493-495.
Gergely, F., Mythily, V., Lee, M., and Raff, J.W. 2003. The ch-Tog/XMAP215 protein is essential for spindle pole organisation in human somatic cells. Genes and Dev. 17, 336-341.|PDF|
2002
Huang, J-Y., and Raff, J.W. 2002. The dynamic localisation of the Drosophila APC/C: evidence for the existence of multiple complexes that perform distinct functions and are differentially localised. J. Cell Sci. 115, 2847-2856.|PDF|
Raff, J.W., Jeffers, K., and Huang, J-Y. 2002. The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time. J. Cell Biol. 157, 1139-1149.|PDF|
Raff, J.W. 2002. Centrosomes and Cancer: lessons from a TACC. Trends Cell Biol. 12, 222-225.
2001
Lee, M.J., Gergely, F., Jeffers, K., Peak-Chew, S-Y., and Raff, J.W. 2001. Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour. Nat. Cell Biol.. 3, 643 – 649.|PDF|
Raff, J.W. 2001. Centrosomes: central no more? Current Biology. 11, R159-R161.
2000
Gergely, F., Karlsson, C., Still, I., Cowell, J., Kilmartin, J., and Raff, J.W. 2000. The TACC domain identifies a new family of centrosomal proteins that can interact with microtubules. Proc. Nat. Acad. Sci. 97, 14352-57.|PDF|
Wakefield, J.G., Huang, J-Y., and Raff, J.W. 2000. A role for centrosomes in regulating the destruction of cyclin B in early Drosophila embryos. Current Biology. 10, 1367-1370.|PDF|
Gergely, F., Kidd, D., Jeffers, K., Wakefield, J.G., and Raff, J.W. 2000. D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo. EMBO J. 19, 241-252.|PDF|