Raff Lab Movie Page
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A WT embryo expressing Tubulin-GFP. Note how dynamic the microtubules are.
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A WT neuroblast expressing Tubulin-GFP. This cell divides asymmetrically.
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The behaviour of DTACC-GFP in a WT embryo. The protein binds to the ends of MTs.
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Centrioles (green) remain roughly centered within the middle of the PCM (red) throughout the cell cycle in WT embryos.
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Centrioles (green) cannot maintain their proper connection to the PCM (red) in cnn mutant embryos, and they rocket around the embryo.
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The rocketing of the centrioles in cnn mutant embryos is dependent on microtubules, and is suppressed by colchicine injection
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The injection of anti-DSas-4 Abs (red) inhibits centriole (green) duplication — most strongly at the bottom right of the embryo, where the Ab concentration is highest.
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The overexpression of DSas-6-GFP leads to extra rounds of centriole duplication within a single cell cycle. Can you see which of these centrioles overduplicates?
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Ncd-GFP is a "plus-tip" protein that binds strongly to spindle microtubules and also to the plus ends of the growing microtubules in this syncytial embryo.
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