Our laboratory has used a group of rat mAb as markers for macrophages in the mouse, during development, in the normal adult and in various disease models. The properties of individual antigens are summarised below. Such a panel of reagents is useful to analyse the presence and phenotype of macrophages in normal and mutant or knock-out mice. For further details see references cited below.
F4/80 restricted to many, but not all mature macrophages. Excellent for foetal and resident macrophages in brain and many non-lymphoid organs. Weakly expressed by blood monocytes and alveolar macrophages. Absent osteoclasts. Also expressed by eosinophils and subsets of dendritic cells.
Macrosialin (FA-11) Pan-macrophage intracellular marker including subpopulations of myeloid-dendritic cells and osteoclasts.
Sialoadhesin (SER-4, 3D6) Subsets of macrophages in bone marrow stroma; subcapsular sinus of lymph nodes and marginal metallophils. Inducible by inflammation and some infections.
Scavenger Receptor A (2F8) Many mature tissue macrophages and hepatic sinusoidal endothelium. Strong on alveolar macrophages, perivascular microglia. Upregulated on microglia by LPS. NB C57BL/6 mice have mutant receptor and reduced 2F8 expression, as determined by immunocytochemistry. Ab 2F8 blocks EDTA-resistant adhesion to serum coated tissue culture plastic.
Mannose receptor (5D3 and others).
Expressed by macrophages and non-vascular endothelium. (Martinez-Pomares
L, Reid, DM, Brown GD, Taylor PR, Stillion RJ, Linehan SA, Zamze S, Gordon S
& Wong SY. Analysis of mannose receptor regulation by IL-4, IL-10, and
proteolytic processing using novel monoclonal antibodies. J. Leukoc. Biol. 2003.
73: 604-613.) Antibody is available through Serotec.
Type 3 Complement Receptor (5C6) Expressed by monocytes, neutrophils and NK cells. Down-regulated tissue macrophages except on microglia. Present on granuloma and inflammatory macrophages. Ab 5C6 blocks myelomonocytic adhesion to serum-coated bacteriologic plastic in vitro and myelomonocytic cell recruitment in vivo, as well as iC3b rosetting.
Ag 7/4. Expressed by PMNs, monocytes and on some
activated macrophages. NB
Polymorphic expression in different mouse strains (Hirsch S
& Gordon S. Polymorphic expression of a neutrophil differentiation
antigen revealed by monoclonal antibody 7/4. Immunogenetics 1983. 18: 229-339.)
Extremely useful for the identification of recruited monocytes during
inflammation (Taylor PR, Brown GD, Geldhof AB, Martinez-Pomares L &
Gordon S. Pattern recognition
receptors and differentiation antigens define murine myeloid cell heterogeneity
ex vivo. Eur. J. Immunol. 2003. 33: 2090-2097;
Henderson RB, Hobbs JA, Mathies M & Hogg N. Rapid recruitment of
inflammatory monocytes is independent of neutrophil migration. Blood 102:
receptor (2A11; Brown GD, Taylor PR, Reid DM, Willment JA, Williams DL, Martinez-Pomares
L, Wong SY & Gordon S. Dectin-1
is a major beta-glucan receptor on macrophages. J. Exp. Med. 2002.
196: 407-412.) Expressed by most macrophages, monocytes, neutrophils,
subsets of dendritic cell and a subset of T cells (Taylor PR,
Brown, GD, Reid DM, Willment JA, Martinez-Pomares L, Gordon S & Wong
SY. The beta-glucan receptor,
dectin-1, is predominantly expressed on the surface of cells of the
monocyte/macrophage and neutrophil lineages. J. Immunol. 2002.
169: 3876-3882.) Dectin-1 is useful for the characterization of
murine myeloid cells. Antibody
is available through Serotec..
For further information contact: Dr.
or Dr. Gordon Brown
or Dr. Gordon Brown
All the above reagents except as noted are available from Serotec:
Serotec Inc: 1017 Main Campus Drive, Suite 2450, Raleigh, North Carolina, 27606, USA.
Customer service tel. no. 919 515 9977 FAX: 919 515 9980
Order hotline toll free 1 800 265 7376
Mr Tim Bernard, SEROTEC, 22 Bankside, Station Approach, Kidlington, Oxford OX5 1JE
Tel: 01865 852700, FAX: 01865 373899
Dainippon Pharmaceuticals Co. Ltd., 33-94, Enokichyo, Suita City, Osaka 564-0053, Japan.
+ 81 6386 2164, FAX: +81 6337 1606
For advice on immunocytochemistry contact Liz Darley and for interpretation contact Siamon Gordon
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